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Principle and Protocol

-For Rat Tissue Sections

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.
Feature
-Histofine® Simple Stain Rat MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded rat tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with rat, human, dog, pig and bovine sera.
Principle & Procedure
Advantages
1. No reaction to endogenous rat immunoglobulin in the tissue (more).
2. Simplified staining steps (more).
3. High sensitivity (more).
4. Unaffected by endogenous biotin in the tissue (more).
5. Ready to use.
Staining Result
- Mouse anti-Vimentin antibody (clone:V9)

Rat colon (treated with high temperature epitope unmasking method) stained with -Histofine® Simple Stain Rat MAX PO (M) and DAB chromogen.
Note cytoplasmic staining of fibroblasts and endothelial cells.
- Mouse anti-PCNA antibody (clone:PC10)

Rat liver stained with -Histofine® Simple Stain Rat MAX PO (MULTI) and DAB chromogen.
Note nuclear staining of liver cell.
- Goat anti-CD3-ε(M-20) antibody

Rat lymph node (treated with high temperature epitope unmasking method) stained with -Histofine® Simple Stain Rat MAX PO(G) and DAB chromogen.
Note membrane staining of almost all lymphocytes.
Product   Detection systems.
Product Name Slides Volume Code For use with
-Histofine® Simple StainTM Rat MAX PO (MULTI) 170 17ml 414191F Mouse and rabbit
primary antibodies
-Histofine® Simple StainTM Rat MAX PO (M) 170 17ml 414171F Mouse
primary antibody
-Histofine® Simple StainTM Rat MAX PO (R) 170 17ml 414181F Rabbit
primary antibody
-Histofine® Simple StainTM Rat MAX PO (G) 170 17ml 414331F Goat
primary antibody
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