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Principle and Protocol

-For Mouse Tissue Sections

 
The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.
Feature
-Histofine® Simple Stain Mouse MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded mouse tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with mouse serum.
Principle & Procedure
Advantages
1. No reaction to endogenous mouse immunoglobulin in the tissue (more).
2. Simplified staining steps (more).
3. High sensitivity (more).
4. Unaffected by endogenous biotin in the tissue (more).
5. Ready to use.
Staining Result
- Rabbit anti-Keratin/Cytokeratin antibody

Mouse skin stained with -Histofine®  Simple Stain Mouse MAX PO(R) and DAB chromogen.
Note cytoplasmic staining of epithelium cells and sweat gland cells.
- Goat anti-CD3-ε (M-20) antibody

Mouse lymph node (treated with high temperature epitope unmasking method) stained with -Histofine®  Simple Stain Mouse MAX PO(G) and DAB chromogen.
Note membrane staining of almost all lymphocytes.
- Rat anti-Mouse CD45R/B220

Mouse spleen staind with -Histofine® Simple Stain Mouse MAX PO (Rat) and DAB chromogen.
Note membrane staining of almost all lymphocytes in germinal center and scattered interfollicular lymphocytes.
Product   Detection systems.
Product Name Slides Volume Code For use with
-Histofine® Simple StainTM Mouse MAX PO (R) 170 17ml 414341F Rabbit
primary antibody
-Histofine® Simple StainTM Mouse MAX PO (G) 170 17ml 414351F Goat
primary antibody
-Histofine® Simple StainTM Mouse MAX PO (Rat) 170 17ml 414311F Rat
primary antibody
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