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Principle and Protocol

-For Human Tissue Sections

The immunohistochemical staining system called Universal Immuno-enzyme Polymer (UIP) method was developed. This is our patented technology.
Feature
-Histofine® Simple Stain MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with human serum.
Principle & Procedure
Advantages
1. Simplified staining steps (more).
2. High sensitivity (more).
3. Unaffected by endogenous biotin in the tissue (more).
4. Ready to use.
Staining Result
- Mouse anti-Progesteron Receptor antibody (clone:1A6)

Human breast cancer (treated with high temperature epitope unmasking method) stained with -Histofine® Simple Stain MAX PO(M) and DAB chromogen.
Note nuclear staining of breast cancer cells.
- Rabbit anti-S-100 protein antibody

Human colon stained with -Histofine® Simple Stain MAX PO(R) and DAB chromogen.
Note cytoplasmic staining of nerve cells.
- Mouse anti-Estrogen Receptor antibody (clone: 1D5)

Human breast cancer (treated with high temperature epitope unmasking method) stained with -Histofine® Simple Stain MAX PO (MULTI) and DAB chromogen.
Note nuclear staining of breast cancer cells.
Product   Detection systems.
Product Name Slides Volume Code For use with Mark in EU
-Histofine® Simple StainTM MAX PO (MULTI) 170 1x17ml 414151F Mouse and rabbit
primary antibodies
CE
500 3x17ml 414152F
1500 9x17ml 414154F
-Histofine® Simple StainTM MAX PO (M) 170 1x17ml 414131F Mouse
primary antibody
500 3x17ml 414132F
1500 9x17ml 414134F
-Histofine® Simple StainTM MAX PO (R) 170 1x17ml 414141F Rabbit
primary antibody
500 3x17ml 414142F
1500 9x17ml 414144F
-Histofine® Simple StainTM MAX PO (G) 170 1x17ml 414161F Goat
primary antibody
 
500 3x17ml 414162F
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